This study will collect blood plasma and white blood cells from individuals using a procedure called apheresis. Apheresis is a method of collecting larger quantities of certain blood components that can safely be collected through a simple blood draw. The blood components will be used in laboratory research studies. Patients 18 years of age and older who are currently enrolled in a NIH clinical research protocol may participate in this study. Relatives of patients and normal healthy volunteers will also be enrolled. Individuals will undergo one of the following two apheresis procedures: - Automated pheresis Blood is drawn through a needle placed in an arm vein and circulated through a cell separator machine. The plasma (liquid part of the blood) and white cells are extracted, and the red cells are re-infused into the donor through the same needle or a needle in the other arm. An anticoagulant (medication to prevent blood from clotting) is usually added to the blood while in the machine to prevent it from clotting during processing. - Manual pheresis One unit (1 pint) of blood is drawn through a needle placed in an arm vein, similar to donating a pint of whole blood. The red blood cells, with or without plasma, are separated from the rest of the blood and returned to the donor through the same needle. Manual pheresis will be done only when a person s estimated total blood volume or red cell count is too low to safely permit removal of blood through a pheresis machine. An adult small in size or markedly anemic, for example, may fall into this category. Some of the blood collected through apheresis may be stored for future studies of HIV disease and immune function and for HLA testing, a genetic test of markers of the immune system. Some of the blood may be used to screen for different types of viral liver infections, such as hepatitis A, B, C, D, E, F, or G.

Population

Community Sample

Brief Title

Apheresis to Obtain Plasma or White Blood Cells for Laboratory Studies

Official Title

Procedures to Obtain Plasma, Lymphocytes, or Other Specimens for Research Studies

Brief Summary

This study will collect blood plasma and white blood cells from individuals using a procedure <br /> called apheresis. Apheresis is a method of collecting larger quantities of certain blood <br /> components that can safely be collected through a simple blood draw. The blood components <br /> will be used in laboratory research studies. <br /> <br /> Patients 18 years of age and older who are currently enrolled in a NIH clinical research <br /> protocol may participate in this study. Relatives of patients and normal healthy volunteers <br /> will also be enrolled. <br /> <br /> Individuals will undergo one of the following two apheresis procedures: <br /> <br /> - Automated pheresis Blood is drawn through a needle placed in an arm vein and circulated <br /> through a cell separator machine. The plasma (liquid part of the blood) and white cells <br /> are extracted, and the red cells are re-infused into the donor through the same needle <br /> or a needle in the other arm. An anticoagulant (medication to prevent blood from <br /> clotting) is usually added to the blood while in the machine to prevent it from clotting <br /> during processing. <br /> <br /> - Manual pheresis One unit (1 pint) of blood is drawn through a needle placed in an arm <br /> vein, similar to donating a pint of whole blood. The red blood cells, with or without <br /> plasma, are separated from the rest of the blood and returned to the donor through the <br /> same needle. Manual pheresis will be done only when a person s estimated total blood <br /> volume or red cell count is too low to safely permit removal of blood through a pheresis <br /> machine. An adult small in size or markedly anemic, for example, may fall into this <br /> category. <br /> <br /> Some of the blood collected through apheresis may be stored for future studies of HIV disease <br /> and immune function and for HLA testing, a genetic test of markers of the immune system. Some <br /> of the blood may be used to screen for different types of viral liver infections, such as <br /> hepatitis A, B, C, D, E, F, or G.

Detailed Description

In order to carry out research procedures on the plasma or mononuclear components of blood,
it is often necessary to obtain larger quantities of plasma or mononuclear cells than can be
safely obtained by simple phlebotomy. These components can be easily and safely obtained
using apheresis procedures in the Clinical Center Apheresis Unit. Other specimens are also
sometimes needed for research, such as other blood components, body fluids (such as semen or
urine) or secretions (from nose, mouth, or different skin areas). While this protocol is
specifically designed to conform to the requirements of the Apheresis Unit for donors to have
apheresis and other collection procedures, it also permits collection of these other donor
specimens. However, the protocol, by itself, is not an independent research study but rather
designed to facilitate sample collections that may aid other studies.

Outcome Measures

Outcome measures are the tests that investigators perform to prove whether or not a treatment being tested in a clinical trial is having any effect.